Box 1 should be stored at -20±5℃.
Box 2 should be stored at 2-8℃.
DNA Circularization Kit is a modular library prep kit designed for MGI high-throughput sequencing platforms. This kit can be used to prepare a single-stranded circular DNA library for MGI sequencers from PCR products with MGI adapters. All reagents provided within this kit have passed stringent quality control and functional verification procedures to ensure high performance, stability, and reproducibility.
Reagent Box | Component | Specification | Quantity |
Box 1 | Splint Buffer | 230 μL | 1 |
DNA Rapid Ligase | 25 μL | 1 | |
Digestion Buffer | 125 μL | 1 | |
Digestion Enzyme | 55 μL | 1 | |
Digestion Stop Buffer | 125 μL | 1 | |
Box 2 | 2nd Strand Enzyme Mix | 1.1 mL | 3 |
Ligation Buffer R | 1.0 mL | 1 |